A combined extraction system for the selective and quantitative determination of the monoamines noradrenaline, adrenaline, dopamine and L-DOPA from the flowers and leaves of Crataegus oxyacantha L. has been developed. The method is based on a preliminary isolation of monoamines by successive petroleum ether and chloroform clean-up, absorption on alumina and passage through Sep-Pak CIS cartridges, followed by measurement of the catecholamines by high pressure liquid chromatography, with electrochemical detection, on a reversed phase column. The described method appears sufficiently simple, sensitive, precise and accurate for routine use and holds great promise for pharmacognostic studies.
THE DETERMINATION OF CATECHOLAMINES IN CRATAEGUS-OXYACANTHA L FLOWERS AND LEAVES BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY ELECTROCHEMICAL DETECTION
SAIJA, Antonina;GERMANO', Maria Paola;CAMPO, Salvatore Giuseppe;DE PASQUALE, Rita
1992-01-01
Abstract
A combined extraction system for the selective and quantitative determination of the monoamines noradrenaline, adrenaline, dopamine and L-DOPA from the flowers and leaves of Crataegus oxyacantha L. has been developed. The method is based on a preliminary isolation of monoamines by successive petroleum ether and chloroform clean-up, absorption on alumina and passage through Sep-Pak CIS cartridges, followed by measurement of the catecholamines by high pressure liquid chromatography, with electrochemical detection, on a reversed phase column. The described method appears sufficiently simple, sensitive, precise and accurate for routine use and holds great promise for pharmacognostic studies.Pubblicazioni consigliate
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