The circadian rhythms have evolved in domestic animals in more different breeding management, and reflect an adaptive mechanism to react in advance to the regular environmental changes. The aim of this research was to evaluate the circadian rhythm of haematological parameters in cows and to study if Brucella abortus infection in cows can alterate their rhythmicity. For this purpose, five healthy cows and five Brucella infected cows were enrolled. Blood samples were collected in spring (sunrise 06:30, sunset 19.00), every 4 h over a 24 h period, starting at 12:00 on day 1 and finishing at 12:00 on day 2, and a complete blood cell count with differential was performed. One-way ANOVA for repeated measures was applied to evaluate the effect of time on haematological parameters. Using cosinor rhythmometry, four rhythmic parameters were determined: mesor, amplitude, acrophase and robustness. One-way repeated measures Analysis of Variance were applied to evaluate a significant effect of time of day on each parameter. P values <0.05 were considered statistically significant. The application of one-way ANOVA showed a statistical significant effect of time of day on haematocrit (PCV) and haemoglobin (Hb) in healthy cows. In Brucella-infected cows, statistical significant changes of neutrophils, eosinophils and lymphocytes were observed. Using Cosinor method, the same parameters showed a daily rhythmicity. Our results showed that a daily rhythmicity exists both for healthy and infected cows, but in different haematological parameters.In particular, hemoglobin, and hematocrit in and neutrophils, eosinophils and lymphocytes in. This study is a preliminary observation in changes of circadian clocks during pathological events in domestic animals. In particular, further investigations should be conducted to evaluate how the molecular clock components are influenced not only by an inflammatory response but contemporarily by a change in L/D cycles.
Influence of Brucella infection on circadian rhythm of haematological parameters in dairy cows
GIUDICE, Elisabetta;MESSINA, VANESSA;MARAFIOTI, SIMONA;BAZZANO, MARILENA;PICCIONE, Giuseppe;
2012-01-01
Abstract
The circadian rhythms have evolved in domestic animals in more different breeding management, and reflect an adaptive mechanism to react in advance to the regular environmental changes. The aim of this research was to evaluate the circadian rhythm of haematological parameters in cows and to study if Brucella abortus infection in cows can alterate their rhythmicity. For this purpose, five healthy cows and five Brucella infected cows were enrolled. Blood samples were collected in spring (sunrise 06:30, sunset 19.00), every 4 h over a 24 h period, starting at 12:00 on day 1 and finishing at 12:00 on day 2, and a complete blood cell count with differential was performed. One-way ANOVA for repeated measures was applied to evaluate the effect of time on haematological parameters. Using cosinor rhythmometry, four rhythmic parameters were determined: mesor, amplitude, acrophase and robustness. One-way repeated measures Analysis of Variance were applied to evaluate a significant effect of time of day on each parameter. P values <0.05 were considered statistically significant. The application of one-way ANOVA showed a statistical significant effect of time of day on haematocrit (PCV) and haemoglobin (Hb) in healthy cows. In Brucella-infected cows, statistical significant changes of neutrophils, eosinophils and lymphocytes were observed. Using Cosinor method, the same parameters showed a daily rhythmicity. Our results showed that a daily rhythmicity exists both for healthy and infected cows, but in different haematological parameters.In particular, hemoglobin, and hematocrit in and neutrophils, eosinophils and lymphocytes in. This study is a preliminary observation in changes of circadian clocks during pathological events in domestic animals. In particular, further investigations should be conducted to evaluate how the molecular clock components are influenced not only by an inflammatory response but contemporarily by a change in L/D cycles.Pubblicazioni consigliate
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