Purpose: e purpose of this study was to evaluate Interferon (IFN)-γ and Interleukin(IL)-17 proles in patients with dierent clinical presentations of pulmonary tuberculosis (TB) and to compare them with those of tuberculin-negative and tuberculinreactive healthy controls Methods: Peripheral blood mononuclear cells (PBMCss), isolated from patients (n=52) and controls (n=30), were stimulated ex vivo with puried protein derivative (PPD) and IFN-γ and IL-17 levels in the supernatant were measured. Results: At baseline, PBMCss from patients with TB released a signicantly lower amount of IL-17 (p=0.043) than PBMCss from healthy controls, whereas IFN-γ levels were similar in the two groups. Aer PPD stimulation, a signicant rise in IL-17 levels was found only among healthy controls (p=0.02). is rise in IL-17 levels was similar between tuberculinreactive and tuberculin-negative subjects. Aer PPD stimulation, patients with inltrative TB secreted higher levels of IL-17 and IFN-γ than those aected with chronic, miliary and cavitary TB (p<0.01). IFN-γ production from patients with inltrative TB was even higher than for healthy controls (p<0.01). PBMCss from tuberculin-reactive patients released higher levels of IFN-γ than tuberculin-negative subjects aer PPD stimulation (p<0.01). Conclusion: Ex vivo PPD stimulation of PBMCs from patients with pulmonary TB does not signicantly stimulate IL-17 release; however, higher IL-17 and IFN-γ levels are found in patients with inltrative disease, in comparison with those aected with miliary, cavitary and chronic TB.

Interferon-γ and interleukin-17 production from PPD-stimulated PBMCss of patients with pulmonary tuberculosis.

Nunnari G;
2013-01-01

Abstract

Purpose: e purpose of this study was to evaluate Interferon (IFN)-γ and Interleukin(IL)-17 proles in patients with dierent clinical presentations of pulmonary tuberculosis (TB) and to compare them with those of tuberculin-negative and tuberculinreactive healthy controls Methods: Peripheral blood mononuclear cells (PBMCss), isolated from patients (n=52) and controls (n=30), were stimulated ex vivo with puried protein derivative (PPD) and IFN-γ and IL-17 levels in the supernatant were measured. Results: At baseline, PBMCss from patients with TB released a signicantly lower amount of IL-17 (p=0.043) than PBMCss from healthy controls, whereas IFN-γ levels were similar in the two groups. Aer PPD stimulation, a signicant rise in IL-17 levels was found only among healthy controls (p=0.02). is rise in IL-17 levels was similar between tuberculinreactive and tuberculin-negative subjects. Aer PPD stimulation, patients with inltrative TB secreted higher levels of IL-17 and IFN-γ than those aected with chronic, miliary and cavitary TB (p<0.01). IFN-γ production from patients with inltrative TB was even higher than for healthy controls (p<0.01). PBMCss from tuberculin-reactive patients released higher levels of IFN-γ than tuberculin-negative subjects aer PPD stimulation (p<0.01). Conclusion: Ex vivo PPD stimulation of PBMCs from patients with pulmonary TB does not signicantly stimulate IL-17 release; however, higher IL-17 and IFN-γ levels are found in patients with inltrative disease, in comparison with those aected with miliary, cavitary and chronic TB.
2013
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11570/3130577
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