Tre casi di studio: le due specie di anemoni di mare Anemonia sulcata e Actinia equina esposti alla Carbamazepina; la specie Anemonia sulcata soggetta a studi tossicologici; studi precedenti sugli effetti degli agenti ambientali in Anthozoa.

Pharmaceuticals and other emerging compounds present in the coastal waters increasingly affects species, not yet all studied. In this study, we have focused attention on species (Anemonia sulcata and Actinia equina) that have been little studied but with an interest in their conservation and biotechnological application. The objectives of this work have been to determine the physiological and biochemical effects of carbamazepine (CBZ) exposure and characterize the degradation routes followed by this compound in water and tissues (biotransformation) of the two anemones species. Ion transport (measured as N, K, ATPase activity), energetic metabolism (measured as glucose and lactate levels) and nitrogen excretion (measured as ammonia concentration in tissues) were determined. Treated samples of A. sulcata and A. equina were analyzed by ultra-high-performance liquid chromatography high- resolution mass spectrometry on a quadrupole-time-of-flight (QqTOF). The structures of nine metabolites have been tentatively identified using HRMS and HRMS/MS data and then confirmed by Medline database program. The current work constitutes the first study on the identification of Cnidaria metabolites of CBZ in species of the Anthozoa Class.