Circadian rhythmicity is an inherent property of living systems and constitutes an essential part of their internal and external temporal order. Changes in circadian rhythms of oxidative stress markers were studied to evaluate the possible involvement of the circadian organization of light/dark cycles on the fluctuation of redox state into three different species. The experimental groups consisted of 5 Ovis aries, 5 Bos taurus and 5 Equus caballus. On all animals, kept under natural photoperiod (sunrise 05:10/sunset 20:45), blood samples were collected on the same days and with the same procedures every 4 h over a 48 h period, starting at 8:00 on day 1 and finishing at 8:00 on day 3. Obtained sera after centrifugation were immediately analyzed by means of a UV spectrophotometer for the assessment of reactive oxygen metabolites (dRoms), antioxidant barrier (Oxy-ads) and thiol-antioxydant barrier (SHp). Two-way measures ANOVA showed a significant effect of time and different species (P<0.0001) and the trigonometric statistical model of the single cosinor procedure indicated the existence of a circadian rhythm of parameters. The oxidative stress parameters showed a mesor value statistically significant in all studied species (P<0.001), a nocturnal acrophase in sheep and diurnal acrophase in cattle and horse. The amplitude was significant on Oxy-ads in horse vs sheep and cattle (P<0.05) instead the robustness was significant on dRoms in horse vs cattle and on SHp in all studied species (P<0.05). In conclusion, we can claim that there is a synergism between oxidative stress markers and the circadian rhythm of anti-oxidant power in different species.
Circadian rhythms of oxidative stress markers in Ovis aries, Bostaurus and Equus caballus
PICCIONE, Giuseppe;FAGGIO, Caterina;TRISCHITTA, Francesca Ross
2010-01-01
Abstract
Circadian rhythmicity is an inherent property of living systems and constitutes an essential part of their internal and external temporal order. Changes in circadian rhythms of oxidative stress markers were studied to evaluate the possible involvement of the circadian organization of light/dark cycles on the fluctuation of redox state into three different species. The experimental groups consisted of 5 Ovis aries, 5 Bos taurus and 5 Equus caballus. On all animals, kept under natural photoperiod (sunrise 05:10/sunset 20:45), blood samples were collected on the same days and with the same procedures every 4 h over a 48 h period, starting at 8:00 on day 1 and finishing at 8:00 on day 3. Obtained sera after centrifugation were immediately analyzed by means of a UV spectrophotometer for the assessment of reactive oxygen metabolites (dRoms), antioxidant barrier (Oxy-ads) and thiol-antioxydant barrier (SHp). Two-way measures ANOVA showed a significant effect of time and different species (P<0.0001) and the trigonometric statistical model of the single cosinor procedure indicated the existence of a circadian rhythm of parameters. The oxidative stress parameters showed a mesor value statistically significant in all studied species (P<0.001), a nocturnal acrophase in sheep and diurnal acrophase in cattle and horse. The amplitude was significant on Oxy-ads in horse vs sheep and cattle (P<0.05) instead the robustness was significant on dRoms in horse vs cattle and on SHp in all studied species (P<0.05). In conclusion, we can claim that there is a synergism between oxidative stress markers and the circadian rhythm of anti-oxidant power in different species.Pubblicazioni consigliate
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