Lysozyme (LZ) concentration, in human and animal species milk, is very different; its antibacterial activity is directed against certain bacterial species. The aim of the present work is to evaluate the antibacterial activity of 3 different donkey milk (DM) bulk samples, on a total of 43 bacterial strains isolated from donkey and bovine milk. The activity was determined by Kirby Bauer (KB) method and by microtiter plates method; MIC was calculated. Bacterial strains showed no susceptibility with KB method. Vice versa, with microtiter plates, milk sample n.1 inhibited only 1 Pseudomonas spp strain (MIC: 5800 U/ml); sample n.2 (MIC:2740 U/ml) never had antibacterial effect; sample n.3 (MIC:3400 U/ml) prevented a 50% of Staphylococcus aureus strains from DM. LZ showed no activity against the bovine milk strains. Finally, the authors underline that a better assessment of LZ antimicrobial activity would be advisable, both for some veterinary health purposes and for consumer (infants with some milk proteins intolerance) security.

STUDIO SULL’ATTIVITA’ ANTIBATTERICA DEL LISOZIMA DEL LATTE D’ASINA. ASPETTI IGIENICO – SANITARI

CONTE, Francesca Laura;FOTI, Maria;GIACOPELLO, CRISTINA;FISICHELLA, Vittorio;
2011

Abstract

Lysozyme (LZ) concentration, in human and animal species milk, is very different; its antibacterial activity is directed against certain bacterial species. The aim of the present work is to evaluate the antibacterial activity of 3 different donkey milk (DM) bulk samples, on a total of 43 bacterial strains isolated from donkey and bovine milk. The activity was determined by Kirby Bauer (KB) method and by microtiter plates method; MIC was calculated. Bacterial strains showed no susceptibility with KB method. Vice versa, with microtiter plates, milk sample n.1 inhibited only 1 Pseudomonas spp strain (MIC: 5800 U/ml); sample n.2 (MIC:2740 U/ml) never had antibacterial effect; sample n.3 (MIC:3400 U/ml) prevented a 50% of Staphylococcus aureus strains from DM. LZ showed no activity against the bovine milk strains. Finally, the authors underline that a better assessment of LZ antimicrobial activity would be advisable, both for some veterinary health purposes and for consumer (infants with some milk proteins intolerance) security.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11570/1917000
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