Amniotic epithelial cells (AECs) are recently considered valid candidates in regenerative medicine and may be useful in cell therapies to treat trauma of tissues with loss of substance. In our study, we molecularly characterized and induced in vitro differentiation of feline AECs, obtained after enzymatic digestion of amnion. The mean doubling time value of AECs was 1,94±0,04 days. By RT-PCR, AECs expressed pluripotent (Oct4, Nanog) and some mesenchymal markers (CD166, CD44), but not MHC-II and CD34. After induction, AECs differentiated into mesodermic and ectodermic lineages, demonstrating high plasticity. In conclusion, feline AECs are pluripotent, weakly immunogenic and may be useful in feline cell therapies.
Molecular characterization and in vitro differentiation of feline amniotic epithelial cells
2012-01-01
Abstract
Amniotic epithelial cells (AECs) are recently considered valid candidates in regenerative medicine and may be useful in cell therapies to treat trauma of tissues with loss of substance. In our study, we molecularly characterized and induced in vitro differentiation of feline AECs, obtained after enzymatic digestion of amnion. The mean doubling time value of AECs was 1,94±0,04 days. By RT-PCR, AECs expressed pluripotent (Oct4, Nanog) and some mesenchymal markers (CD166, CD44), but not MHC-II and CD34. After induction, AECs differentiated into mesodermic and ectodermic lineages, demonstrating high plasticity. In conclusion, feline AECs are pluripotent, weakly immunogenic and may be useful in feline cell therapies.Pubblicazioni consigliate
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