Aquatic organisms are exposed to various kinds of environmental pollutants having different effects. Metals, such as cadmium, are released from various anthropogenic sources and can disrupt many biological functions. Mussels are largely used in biomonitoring studies because they are known to be efficient accumulators of contaminants and good bioindicators. They are able to accumulate high amounts of Cd2+ in their tissues such as gills and digestive gland. Cd2+ also induces endocrine disruption and increases reactive oxygen species (ROS) production. It has recently been established that the use of proteomics can be an useful tool in the field of ecotoxicology. Proteomic techniques enable the detection of changes of protein expression. Protein expression profiles were obtained in the cytosolic fraction of digestive glands of Mytilus galloprovincialis unexposed and exposed to CdCl2 (40μgL-1) for 21 days using two-dimension electrophoresis. Protein spots were detected with the PD-Quest analysis software. CdCl2 induced significant alterations: the expression of 47 new proteins and the suppression of 143. 30 proteins were two-fold up-regulated after CdCl2 exposure, while 41 proteins were down-regulated (2-fold). Our results confirm the validity of the proteomic analysis to detect the molecular effect of subletal concentration of Cd on a bioindicator organism. The next step of this work will be to identify the differently expressed protein by MS-MS.
Changes in the Mytilus galloprovincialis proteome after CdCl2 exposure
TORRE, AGATA;TRISCHITTA, Francesca Ross;
2012-01-01
Abstract
Aquatic organisms are exposed to various kinds of environmental pollutants having different effects. Metals, such as cadmium, are released from various anthropogenic sources and can disrupt many biological functions. Mussels are largely used in biomonitoring studies because they are known to be efficient accumulators of contaminants and good bioindicators. They are able to accumulate high amounts of Cd2+ in their tissues such as gills and digestive gland. Cd2+ also induces endocrine disruption and increases reactive oxygen species (ROS) production. It has recently been established that the use of proteomics can be an useful tool in the field of ecotoxicology. Proteomic techniques enable the detection of changes of protein expression. Protein expression profiles were obtained in the cytosolic fraction of digestive glands of Mytilus galloprovincialis unexposed and exposed to CdCl2 (40μgL-1) for 21 days using two-dimension electrophoresis. Protein spots were detected with the PD-Quest analysis software. CdCl2 induced significant alterations: the expression of 47 new proteins and the suppression of 143. 30 proteins were two-fold up-regulated after CdCl2 exposure, while 41 proteins were down-regulated (2-fold). Our results confirm the validity of the proteomic analysis to detect the molecular effect of subletal concentration of Cd on a bioindicator organism. The next step of this work will be to identify the differently expressed protein by MS-MS.Pubblicazioni consigliate
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