Factor H binding protein (fHbp) of N.meningitidis is a down-regulatory molecule in the complement cascade, and binding of this molecule to the bacterial surface contributes to the ability of the organism to avoid complement-mediated killing. Bacteriophage  has emerged as an effective vehicle for the surface display of complex repertoires from natural sources, alternative to the more common filamentous phage systems. We have screened 3 libraries, displaying V.1, V.2 and V.3 fHbp fragments from N. meningitidis, with 5 anti-fHbp mAbs from mice immunized with V.2 or V.3 rfHbp proteins to identify fragments of fHbp involved in the binding of fH. Specific phage clones were isolated and sequenced. Multiple sequence alignment allowed us to identify specific amino acid residues corresponding to the mAb reactivity patterns.

Screening of gene-specific libraries of Neisseria meningitidis fHbp displayed on bacteriophage lambda capsid

LO PASSO, Carla;PERNICE, Ida;GALBO, Roberta;BENINATI, Concetta;
2012-01-01

Abstract

Factor H binding protein (fHbp) of N.meningitidis is a down-regulatory molecule in the complement cascade, and binding of this molecule to the bacterial surface contributes to the ability of the organism to avoid complement-mediated killing. Bacteriophage  has emerged as an effective vehicle for the surface display of complex repertoires from natural sources, alternative to the more common filamentous phage systems. We have screened 3 libraries, displaying V.1, V.2 and V.3 fHbp fragments from N. meningitidis, with 5 anti-fHbp mAbs from mice immunized with V.2 or V.3 rfHbp proteins to identify fragments of fHbp involved in the binding of fH. Specific phage clones were isolated and sequenced. Multiple sequence alignment allowed us to identify specific amino acid residues corresponding to the mAb reactivity patterns.
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11570/2468621
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact