Coupled LC-GC to determine the sesquiterpene fraction of Cannabis sativa var. codimono essential oil.

This research studies Cannabis sativa var. codimono essential oil, whose volatile fraction, containing monoterpenes and sesquiterpenes, has a peculiar fragrance. Sesquiterpene fraction was studied by means of a LC-GC coupling method. This technique allowed to differentiate the sesquiterpenes from other classes of interfering compounds that can make the identification and the determination more difficult. The technique involves the introduction of the essential oil into the liquid chromatography, where separation of the terpene fraction from the other classes of compounds available in the oil, occurs. This fraction is transferred on-line to the GC, and this enables various sesquiterpenes are separated without other interference. The method allowed to determine with greater security longifolene, β-caryophyllene, α-humulene, α-bergamottene, β- bisabolene and caryophyllene oxide.