In the last decade there was an increased incidence of leishmaniasis associated with a geographic spread from areas traditionally endemic to areas previously considered not endemic. It is obviously linked to the spread and survival of Phlebotomus perniciosus, main vector of leishmaniasis in Italy. Concomitant factors including phenomena such as stray dogs and the changed environmental climate were involved on spread of leishmaniasis. The increase of human cases and the incidence in the territory has meant that in control thereof have been realized in Italy several plans for monitoring and surveillance, last of these has been implemented in the Calabria region. A control plan of canine leishmaniasis was performed by Calabria Region (Decree 181 of 28 November 2012) in the years ranging from 2010 to 2014. Four shelter located in the Province of Vibo Valentia were monitored. Five hundred-sixty- nine subjects of three public shelter (Municipal Doghouse of Vibo Valentia, Oasi Canina and Mondo di Pluto) and five hundred-fifty-four dogs were tested by IFAT (Immunofluores- cence AntibodyTest ). The presence of anti-L. infantum antibodies was detected by indirect immunofluorescence antibody test (IFAT) in according to the recommendation of OIE using MHON/TN/80/IPT1 as a whole-parasite antigen fixed on multispot slides (Bio Merieux Spa, Florence, Italy) and fluorescently labelled anticanine gamma globulin (Sigma Aldrich, Milan; Italy) as conjugate. Positive and negative controls were included on each work session. Positive sera were serially diluted and tested to establish the maximum reaction titer. A dilution 1:160 was considered positive. The results showed an higher prevalence to IFAT of subjects owned than the dogs housed in kennel. The 46% (n. 181/388 cases) of dogs owned found positive to IFAT. Only 10.1 % (n. 51/503 cases )of dogs living in the shelter was positive. The breed belonged or a different immune response th1 Vs th2 may be relate to the different positivity detected on subjects examined in our study.
MONITORING OF CANINE LEISHMANIASIS IN THE PROVINCE OF VIBO VALENTIA (ITALY, CALABRIA): DIFFERENT DISTRIBUTION BETWEEN DOGS WITH OWNER AND POPULATION FROM DOG POUND
PUGLIESE, Michela;MIGLIAZZO, ANTONELLA;DE MAJO, Massimo;PUGLIESE, Antonio
2015-01-01
Abstract
In the last decade there was an increased incidence of leishmaniasis associated with a geographic spread from areas traditionally endemic to areas previously considered not endemic. It is obviously linked to the spread and survival of Phlebotomus perniciosus, main vector of leishmaniasis in Italy. Concomitant factors including phenomena such as stray dogs and the changed environmental climate were involved on spread of leishmaniasis. The increase of human cases and the incidence in the territory has meant that in control thereof have been realized in Italy several plans for monitoring and surveillance, last of these has been implemented in the Calabria region. A control plan of canine leishmaniasis was performed by Calabria Region (Decree 181 of 28 November 2012) in the years ranging from 2010 to 2014. Four shelter located in the Province of Vibo Valentia were monitored. Five hundred-sixty- nine subjects of three public shelter (Municipal Doghouse of Vibo Valentia, Oasi Canina and Mondo di Pluto) and five hundred-fifty-four dogs were tested by IFAT (Immunofluores- cence AntibodyTest ). The presence of anti-L. infantum antibodies was detected by indirect immunofluorescence antibody test (IFAT) in according to the recommendation of OIE using MHON/TN/80/IPT1 as a whole-parasite antigen fixed on multispot slides (Bio Merieux Spa, Florence, Italy) and fluorescently labelled anticanine gamma globulin (Sigma Aldrich, Milan; Italy) as conjugate. Positive and negative controls were included on each work session. Positive sera were serially diluted and tested to establish the maximum reaction titer. A dilution 1:160 was considered positive. The results showed an higher prevalence to IFAT of subjects owned than the dogs housed in kennel. The 46% (n. 181/388 cases) of dogs owned found positive to IFAT. Only 10.1 % (n. 51/503 cases )of dogs living in the shelter was positive. The breed belonged or a different immune response th1 Vs th2 may be relate to the different positivity detected on subjects examined in our study.Pubblicazioni consigliate
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