Susceptibility of erythrocytes from different sources to xenobiotics-induced lysis

As erythrocytes are continuously exposed to plenty of metabolites and toxicants, the aim of the present work is to show whether erythrocytes from different sources may exhibit different susceptibility to hemolysis induced by two classes of xenobiotics, Cnidaria venoms, acting via pore forming mechanism on cell membrane, and Cd2+, inducing cell damage mainly via lipid peroxidation. To this end, the hemolytic power of crude venom from stinging cells of the anthozoan Aiptasia mutabilis and from the scyphozoan Pelagia noctiluca has been tested, along with Cd2+ (5 to 20 mM), on erythrocytes (0.05% v/v) obtained from either rabbit, or dog, or chicken, or human source. Our results show a more significant susceptibility of rabbit erythrocytes to hemolysis induced by both crude venom and Cd2+ than erythrocytes from other sources. This difference seems to rely neither on the different specimens used to extract crude venom, nor on the different mechanism of toxicants. In this light, the present study may contribute: i) to confirm hemolytic test as a suitable biological assay to verify erythrocytes resistance to toxicants; ii) to show variability in hemolytic response to xenobiotics; iii) to propose rabbit erythrocytes as more sensitive to the lytic action of xenobiotics, adding more knowledge about clinical practice and drug development in these animals.