The occurrence of new pandemic viruses, such as SARS-CoV-1 (2003), MERS-CoV (2012) and SARS-CoV-2 (2019) has indicated an urgent need for diagnostic tools able to reliably identify infected individuals and to determine if the infection is in the acute phase. Although nanotechnology based on graphene has been poorly applied for the rapid diagnosis of viral diseases, the extraordinary properties of graphene have been recently exploited for the diagnosis of COVID-19. Novel graphene-based field-effect transistor (GFET) biosensors were developed for the quantitative detection of viral RNA and viral spike protein. The fabrication of COVID-19 FET sensor for spike protein recognition is based on the integration of the SARS-CoV-2 spike antibody with graphene, whereas the GFET sensor for viral RNA recognition exploited the CRISPR/Cas biotechnology.
Graphene-Based Strategies in the Diagnosis of Viral Diseases
Scala, Angela;Cordaro, Annalaura;Neri, Giulia;Sciortino, Maria Teresa;Piperno, Anna
2020-01-01
Abstract
The occurrence of new pandemic viruses, such as SARS-CoV-1 (2003), MERS-CoV (2012) and SARS-CoV-2 (2019) has indicated an urgent need for diagnostic tools able to reliably identify infected individuals and to determine if the infection is in the acute phase. Although nanotechnology based on graphene has been poorly applied for the rapid diagnosis of viral diseases, the extraordinary properties of graphene have been recently exploited for the diagnosis of COVID-19. Novel graphene-based field-effect transistor (GFET) biosensors were developed for the quantitative detection of viral RNA and viral spike protein. The fabrication of COVID-19 FET sensor for spike protein recognition is based on the integration of the SARS-CoV-2 spike antibody with graphene, whereas the GFET sensor for viral RNA recognition exploited the CRISPR/Cas biotechnology.Pubblicazioni consigliate
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