The development of novel nanomedicines for controlled release of phototherapeutic agents is a highly investigated research topic. Antimicrobial photodynamic therapy (aPDT) is a successful approach to treat bacterial infections that are resistant to antibiotics. Following our recent studies on nanophototherapeutics [1], here we propose the design and characterization of a novel photosensitizing nanosystem based on the trade cyclodextrin CAPTISOL® (sulphobutylether-beta-cyclodextrin, SBE-beta-CD) and meso-tetrakis(N-methylpyridinium- 4-yl)porphine (TMPyP) as potential aPDT tool. Firstly, CAPTISOL®/ TMPyP nanocomplexes were prepared at 1:1 stoichiometry and characterized by UV/vis, fluorescence spectroscopy, Dynamic Light Scattering (DLS), -potentialmeasurements, AFM and scanning-near field optical luminescence (SNOL), thus elucidating stability constants, photophysical, morphological and release kinetic properties. Finally, photoantimicrobial activity of the CAPTISOL®/TMPyP vs free TMPyP was investigated against Gram-negative P. aeruginosa and E. coli and Gram-positive S. aureus, showing the designed nanosystems can control the time-release of porphyrin to photokill bacterial cells.

NANOASSEMBLIES BASED ON CAPTISOL®/PORPHYRIN COMPLEXES WITH ANTIMICROBIAL PHOTODYNAMIC ACTION

Roberto Zagami
;
2020

Abstract

The development of novel nanomedicines for controlled release of phototherapeutic agents is a highly investigated research topic. Antimicrobial photodynamic therapy (aPDT) is a successful approach to treat bacterial infections that are resistant to antibiotics. Following our recent studies on nanophototherapeutics [1], here we propose the design and characterization of a novel photosensitizing nanosystem based on the trade cyclodextrin CAPTISOL® (sulphobutylether-beta-cyclodextrin, SBE-beta-CD) and meso-tetrakis(N-methylpyridinium- 4-yl)porphine (TMPyP) as potential aPDT tool. Firstly, CAPTISOL®/ TMPyP nanocomplexes were prepared at 1:1 stoichiometry and characterized by UV/vis, fluorescence spectroscopy, Dynamic Light Scattering (DLS), -potentialmeasurements, AFM and scanning-near field optical luminescence (SNOL), thus elucidating stability constants, photophysical, morphological and release kinetic properties. Finally, photoantimicrobial activity of the CAPTISOL®/TMPyP vs free TMPyP was investigated against Gram-negative P. aeruginosa and E. coli and Gram-positive S. aureus, showing the designed nanosystems can control the time-release of porphyrin to photokill bacterial cells.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11570/3182060
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