2-Pentadecyl-2-oxazoline (PEAOXA): an anti-inflammatory and anti-nociceptive compound and its impact in the marine environment in a model of Zebrafish embryos and larvae (Danio rerio)

Palmitoylethanolamide-Oxazoline (PEA-OXA), is a palmitoylethanolamide derivative with anti-inflammatory and analgesic actions. Also present in nature such as in chocolate, PEA-OXA has demonstrated excellent therapeutic effects in numerous models of inflammation. Exogenous administration of PEA-OXA could be a useful alternative to enhance the endogenous protective mechanisms set up by the body in response to different types of damage. The mechanism of action of PEA-OXA is still under study, several hypotheses could justify its anti-inflammatory action, one of all the ability to modulate the action of the enzyme NAAA, responsible for the degradation of PEA, through the benzene ring, thus increasing the endogenous levels of PEA and therefore its targeted action in the regulation of inflammatory processes and pain. The aim of my PhD project was to study the action of PEA-OXA, using the animal model of zebrafish (Danio rerio), during the early stages of development in the embryonic stage and later in larvae, in order to analyze the anti-inflammatory and analgesic effects both under physiological conditions and following the presence of inflammatory agents, such as LPS (Lipopolysaccharide) or DSS (Dextran Sodium Sulfano), or nociceptive agents such as Acetic Acid.