Human erythrocytes during their lifespan of 120 ± 20 days, are exposed to a large number of stressful situations. Several erythrocyte parameters can be negatively affected by increased oxidative stress (OS). This study was conducted to evaluate erythrocyte-triggered response against OS after 20 mM H2O2 treatment and consequently protective role of Quercetin (Q), a polyphenolic flavonoid compound, known for its antioxidant properties. In this regard, % hemolysis, cell shape, reactive oxygen species (ROS) production, thiobarbituric acid reactive substances (TBARS) levels, oxidation of protein sulfhydryl groups (-SH), CD47 and Band 3 protein (B3p) expression, % methemoglobin (% MetHb), anion exchange capability through B3p, have been analysed in erythrocytes treated with 20 mM H2O2 for 1 hour, with or without 10 μM Q pre-treatment (1 hour) at 25 °C. Our data show that: pre-treatment with 10 μM Q avoided the morphological alterations (acanthocytes); prevented H2O2-induced OS damage; and restored the abnormal distribution of B3p and CD47. Moreover, H2O2 exposure was associated with a decrease of the rate constant of SO4²⁻ uptake, as well as % MetHb formation. Both alterations have been attenuated by pre-treatment with 10 μM Q. These results contribute to elucidate OS processes in human erythrocytes and propose Q as natural antioxidant for the treatment and prevention of OS-related disease conditions impacting on erythrocytes.

Morphological and Functional Alterations in H2O2-treated Erythrocytes: Role of Quercetin

A. Remigante;S. Spinelli;D. Caruso;Angela Marino;R Morabito
2022-01-01

Abstract

Human erythrocytes during their lifespan of 120 ± 20 days, are exposed to a large number of stressful situations. Several erythrocyte parameters can be negatively affected by increased oxidative stress (OS). This study was conducted to evaluate erythrocyte-triggered response against OS after 20 mM H2O2 treatment and consequently protective role of Quercetin (Q), a polyphenolic flavonoid compound, known for its antioxidant properties. In this regard, % hemolysis, cell shape, reactive oxygen species (ROS) production, thiobarbituric acid reactive substances (TBARS) levels, oxidation of protein sulfhydryl groups (-SH), CD47 and Band 3 protein (B3p) expression, % methemoglobin (% MetHb), anion exchange capability through B3p, have been analysed in erythrocytes treated with 20 mM H2O2 for 1 hour, with or without 10 μM Q pre-treatment (1 hour) at 25 °C. Our data show that: pre-treatment with 10 μM Q avoided the morphological alterations (acanthocytes); prevented H2O2-induced OS damage; and restored the abnormal distribution of B3p and CD47. Moreover, H2O2 exposure was associated with a decrease of the rate constant of SO4²⁻ uptake, as well as % MetHb formation. Both alterations have been attenuated by pre-treatment with 10 μM Q. These results contribute to elucidate OS processes in human erythrocytes and propose Q as natural antioxidant for the treatment and prevention of OS-related disease conditions impacting on erythrocytes.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11570/3239925
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