Efficacy of a novel therapeutic, based on natural ingredients and probiotics, in a murine model of multiple food intolerance and maldigestion

Patients with hypersensitive gut mucosa often suffer from food intolerances associated with an inadequate gastrointestinal function that affects 15-20% of the population. Current treatments involve elimination diets, but require careful control, are difficult to maintain long-term and diagnosis remains challenging. This study aims to evaluate the beneficial effects of a novel therapeutic of natural (NTN) origin containing food-grade polysaccharides, proteins, and grape seed extract to restore intestinal function in a murine model of fructose, carbohydrate, and fat intolerances. All experiments were conducted in 4-week-old male CD1 mice. To induce food intolerances mice were fed with either a high-carbohydrate diet (HCD), high-fat diet (HFD), or high-fructose diet (HFrD) respectively. After two weeks of treatment, several parameters and endpoints were evaluated such as: food and water intake, body weight, histological score in several organs, gut permeability, intestinal epithelial integrity, and biochemical endpoints. Our results demonstrated that the therapeutic agent significantly restored gut barrier integrity and permeability compromised by every food intolerance induction. Restoration of intestinal function by NTN treatment has consequently improved tissue damage in several functional organs involved in the diagnosis of each intolerance such as the pancreas for HCD and the liver for HFD and HFrD. Taken together, our results support NTN as promising natural care in the non-pharmacological strategy for the recovery of intestinal dysregulation, supporting the well-being of the gastrointestinal tract.