Despite the early gloomy prospects for the development of a suitable interface and operation, instrumental couplings of liquid chromatography (LC) to mass spectrometry (MS) have experienced an enormous growth in the last two decades; the number of applications of this powerful hyphenated technique have exponentially increased, consistently. Arguably, the advances made in miniaturized LC systems and column technology have strongly prompted such an evolution; as a matter of fact, capillary LC-MS (and tandem MS) nowadays accounts for the vast majority of applications performed, e.g., through “omics” technologies. Today any mass spectrometer potentially can be hyphenated to an LC separation system by properly interfacing the chromatographic and spectrometric components; this was a major technical challenge earlier. Understanding the working principles and technical properties of different MS instruments gives an insight into the technical possibilities and limitations to be acknowledged when coupling MS with LC. In the sections that follow, the readers will be given theoretical and practical guidance on the analytical capabilities of most common types of MS analyzers, which constrain the utility of an instrument for handling the specific analytical task at hand.
Theoretical and practical aspects of LC-MS analysis
Donato P.;Arena P.;Mondello L.
2019-01-01
Abstract
Despite the early gloomy prospects for the development of a suitable interface and operation, instrumental couplings of liquid chromatography (LC) to mass spectrometry (MS) have experienced an enormous growth in the last two decades; the number of applications of this powerful hyphenated technique have exponentially increased, consistently. Arguably, the advances made in miniaturized LC systems and column technology have strongly prompted such an evolution; as a matter of fact, capillary LC-MS (and tandem MS) nowadays accounts for the vast majority of applications performed, e.g., through “omics” technologies. Today any mass spectrometer potentially can be hyphenated to an LC separation system by properly interfacing the chromatographic and spectrometric components; this was a major technical challenge earlier. Understanding the working principles and technical properties of different MS instruments gives an insight into the technical possibilities and limitations to be acknowledged when coupling MS with LC. In the sections that follow, the readers will be given theoretical and practical guidance on the analytical capabilities of most common types of MS analyzers, which constrain the utility of an instrument for handling the specific analytical task at hand.Pubblicazioni consigliate
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