Corrigendum to “Therapeutic potential of dinitrobenzene sulfonic acid (DNBS)-induced colitis in mice by targeting IL-1β and IL-18’’ [Biochem. Pharmacol. 155 (2018) 150–161, (S0006295218302569), (10.1016/j.bcp.2018.06.029)]

The authors regret that they inadvertently duplicated the insets L1 and M1 of Figure 5, and the NF-kBp65 blot of Figure 8A with the p-p38 blot of Figure 9B. They apologize for any inconvenience these errors may have caused and wish to assure their commitment to upholding the integrity and accuracy of the research findings. The conclusions of the article remain valid and are not affected by these mistakes. The correct versions of Figures 5 and 9 are provided below together with a revised version of section 2.10 about the Western blot protocols used in the research.[Formula presented] Fig. 5. The genetic lack of IL1β/IL18 on CD4+ and CD8+ expression after DNBS injection. Immunohistochemical analysis for CD4+ and CD8+ in sham WT (A, A1, G, G1), in DNBS WT (B, B1, H, H1), in DNBS WT treated with Anakinra (C, C1, I, I1), in DNBS IL-18 KO (D, D1, L, L1) and in DNBS double IL-1β/IL18 KO mice (E, E1, M, M1). The percentage of positive staining (% brown staining) as a function of total tissue area is given in panels F, N. Images are representative of at least three experiments performed on different days. ND: not detectable. Values are means ± SD of 10 animals for each group; ***P < 0.001 vs Sham, ##P < 0.01 vs DNBS WT ###P < 0.001 vs DNBS WT, ***P < 0.001 vs DNBS WT + Anakinra.[Formula presented] Fig. 9. The genetic lack of IL1β/IL18 on MAPKs pathway and IFN-γ release after DNBS injection. Representative western blots for p-ERK (A) and p-p38 expression (B) and IFN-γ (C) were performed. In addition, mRNA levels of IFN-γ were also examinated (D). Shown is a representative blot from 10 animals/group, together with a densitometric analysis for all animals. The results in A, B C, D are expressed as means ± SD of 10 animals for each group. **P < 0.01 vs Sham, ***P < 0.001 vs Sham, #P < 0.05 vs DNBS WT, ##P < 0.01 vs DNBS WT, ###P < 0.001 vs DNBS WT. 2.10. Western blots for IKB-α, NF-κBp65, COX-2, phospho-ERK, phospho-p38 and IFN-γ Cytosolic and nuclear extracts were prepared as previously described [20]. The following primary antibodies were used: anti-IκB-α (1:500; Santa Cruz Biotechnology, DBA Italia srl, Milan Italy), anti-NF-κB p65 (1:500; Santa Cruz Biotechnology, DBA Italia srl, Milan Italy), anti-COX-2 (1:500; Santa Cruz Biotechnology, DBA Italia srl, Milan Italy), anti- p-p38 (1:500, Cell Signaling DBA Italia srl, Milan Italy) anti-p-ERK (1:500; Santa Cruz Biotechnology, DBA Italia srl, Milan Italy) and anti-IFN-γ (1:100, R&D Systems, Minneapolis MN, USA). To determine that blots were loaded with equal volumes of lysate, they were also probed with anti-β-actin or anti-lamin A/C antibodies. The membranes were stripped by agitation with 2% glycine, pH 2, blocked in 5% non-fat dried milk/PBS for 1 hour at RT and re-incubated several times with different antibodies to optimize the detection of the proteins minimizing the number of gels and transfers. Relative expression of protein bands (IKB-α, 37 kDa; NF-κB p65, 65 kDa; COX-2, 72 kDa, p-p38 43 kDa, p-ERK 46–54 kDa) was detected with a chemiluminescence detection system reagent according to the manufacturer's instructions (Super Signal West Pico Chemiluminescent Substrate; Pierce). Densitometry was quantified with BIORAD ChemiDocTM XRS + software and standardized to β-actin or lamin A/C levels. Images of blot signals (8 bit/600 dpi resolution) were imported to analysis software (Image Quant TL, v2003). Daniela Impellizzeri: Methodology, Writing – original draft. Rosalba Siracusa: Methodology. Marika Cordaro: Methodology. Alessio Filippo Peritore: Methodology. Enrico Gugliandolo: Data curation, Formal analysis. Giuseppe Mancuso: Data curation, Formal analysis. Angelina Midiri: Data curation, Formal analysis. Rosanna Di Paola: Writing – review & editing. Salvatore Cuzzocrea: Conceptualization, Supervision.