OBJECTIVE: The preliminary study was carried out to determine the occurrence of MRSA in small ruminants in milk farms in Sicily. MATERIALS AND METHODS: A total of 257 samples (n. 138 nasal swabs and n. 119 milk samples) was collected in 15 farms from 171 sheep and 86 goats. All samples were processed by conventional method using Nutrient Broth and Baird Parker Agar. Staphylococci were identified by biochemical tests (Api Staph System; BioMérieux). Antimicrobial susceptibility testing of all isolates was performed on Mueller-Hinton agar plates by Kirby Bauer's Disc Diffusion method. The identified Staphylococcus aureus (Sa) strains were subjected to a PCR protocol for the detection of the mecA gene. RESULTS: Out of 267 Staphylococcus spp strains isolated 101 (37,8 %) (37 coagulase positive and 64 coagulase negative) were methicillin-oxacillin resistant. Out of coagulase + strains, n.18 strains were identified as Sa. Despite Sa isolates were found as methicillin-resistant, only 1 strain carried a mecA gene. CONCLUSIONS: Our results let us to suppose a wide spread of. methicillin resistant Staphylococcus spp in Sicilian flocks. Humans could acquire antibiotic resistant bacteria through milk consumption. Risk factors for these infections in animals are currently under investigation and literature data are essential for the definition of specific guidelines for consumer health protection

Methicillin-resistant Staphylococcus aureus (MRSA) in Sicilian sheep and goats.

FOTI, Maria;RINALDO, Donatella;PASSANTINO, Annamaria;FISICHELLA, Vittorio;GIACOPELLO, CRISTINA;CONTE, Francesca Laura
2009

Abstract

OBJECTIVE: The preliminary study was carried out to determine the occurrence of MRSA in small ruminants in milk farms in Sicily. MATERIALS AND METHODS: A total of 257 samples (n. 138 nasal swabs and n. 119 milk samples) was collected in 15 farms from 171 sheep and 86 goats. All samples were processed by conventional method using Nutrient Broth and Baird Parker Agar. Staphylococci were identified by biochemical tests (Api Staph System; BioMérieux). Antimicrobial susceptibility testing of all isolates was performed on Mueller-Hinton agar plates by Kirby Bauer's Disc Diffusion method. The identified Staphylococcus aureus (Sa) strains were subjected to a PCR protocol for the detection of the mecA gene. RESULTS: Out of 267 Staphylococcus spp strains isolated 101 (37,8 %) (37 coagulase positive and 64 coagulase negative) were methicillin-oxacillin resistant. Out of coagulase + strains, n.18 strains were identified as Sa. Despite Sa isolates were found as methicillin-resistant, only 1 strain carried a mecA gene. CONCLUSIONS: Our results let us to suppose a wide spread of. methicillin resistant Staphylococcus spp in Sicilian flocks. Humans could acquire antibiotic resistant bacteria through milk consumption. Risk factors for these infections in animals are currently under investigation and literature data are essential for the definition of specific guidelines for consumer health protection
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11570/4679
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