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EnglishA seminested PCR typing assay has been extended to identify rotavirus strains with the P[14] genotype. The specificity of the method was confirmed by Southern hybridization and by restriction analysis with the enzyme AluI. One out of four human rotavirus (HRV) strains with unusual subgroup-electropherotype linkage but none out of 50 HRV strains with usual linkage was typed as P[14].
| Titolo: | Identification of human rotavirus strains with the P[14] genotype by PCR |
| Autori: | |
| Data di pubblicazione: | 1999 |
| Rivista: | |
| Abstract: | A seminested PCR typing assay has been extended to identify rotavirus strains with the P[14] genotype. The specificity of the method was confirmed by Southern hybridization and by restriction analysis with the enzyme AluI. One out of four human rotavirus (HRV) strains with unusual subgroup-electropherotype linkage but none out of 50 HRV strains with usual linkage was typed as P[14]. |
| Handle: | http://hdl.handle.net/11570/12895 |
| Appare nelle tipologie: | 14.a.1 Articolo su rivista |
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