The present study was aimed at evaluating the in vitro antioxidant activity and in vivo photoprotective activity of an extract of wine obtained from Jacquez (Vitis aestivalis-cinerea x V vinifera) grapes (JW-E). The chemical profile of the JW-E was characterised by a significant level of proanthocyanidins, together with lower amounts of anthocyanins and hydroxycinnamic acids. The antioxidant activity of the JW-E was assessed by means of various in vitro tests (bleaching of the stable 1,1-diphenyl-2-picrylhydrazyl radical; peroxidation, induced by the water-soluble radical initiator 2,2′-azobis(2-amidinopropane) hydrochloride, on mixed dipalmitoylphosphatidylcholine/linoleic acid unilamellar vesicles; UV radiation-induced peroxidation in phosphatidylcholine multilamellar vesicles). In all in vitro tests employed, the JW-E proved to possess strong antioxidant/free radical-scavenging effectiveness. Furthermore, when topically applied, a gel formulation containing the JW-E afforded significant in vivo protection against UVB light-induced skin erythema (monitored by reflectance spectrophotometry) in healthy human volunteers.
Chemical analysis and photoprotective effect of an extract of wine from Jacquez grapes
TOMAINO, Antonio;CIMINO, Francesco;SAIJA, Antonina
2002-01-01
Abstract
The present study was aimed at evaluating the in vitro antioxidant activity and in vivo photoprotective activity of an extract of wine obtained from Jacquez (Vitis aestivalis-cinerea x V vinifera) grapes (JW-E). The chemical profile of the JW-E was characterised by a significant level of proanthocyanidins, together with lower amounts of anthocyanins and hydroxycinnamic acids. The antioxidant activity of the JW-E was assessed by means of various in vitro tests (bleaching of the stable 1,1-diphenyl-2-picrylhydrazyl radical; peroxidation, induced by the water-soluble radical initiator 2,2′-azobis(2-amidinopropane) hydrochloride, on mixed dipalmitoylphosphatidylcholine/linoleic acid unilamellar vesicles; UV radiation-induced peroxidation in phosphatidylcholine multilamellar vesicles). In all in vitro tests employed, the JW-E proved to possess strong antioxidant/free radical-scavenging effectiveness. Furthermore, when topically applied, a gel formulation containing the JW-E afforded significant in vivo protection against UVB light-induced skin erythema (monitored by reflectance spectrophotometry) in healthy human volunteers.Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.