Among disaccharides, trehalose, a naturally-occurring glass-forming disaccharide, is particularly effective in terms of its ability to preserve several organisms under stress conditions. The present work is aimed to investigate the bioprotective action of trehalose on lysozyme as a function of temperature. In order to extract quantitative information about the dimensional changes of the proteins, we performed Small Angle Neutron Scattering measurements on lysozyme/D2O solutions in absence and in presence of the disaccharide. The findings emphasise the capability of trehalose to stabilise the protein, activating aggregationprocesses.

Aggregation processes of biomolecules in presence of trehalose

MAGAZU', Salvatore;MIGLIARDO, Federica;BARRECA, Davide;BELLOCCO, Ersilia Santa;LAGANA', Giuseppina
2007-01-01

Abstract

Among disaccharides, trehalose, a naturally-occurring glass-forming disaccharide, is particularly effective in terms of its ability to preserve several organisms under stress conditions. The present work is aimed to investigate the bioprotective action of trehalose on lysozyme as a function of temperature. In order to extract quantitative information about the dimensional changes of the proteins, we performed Small Angle Neutron Scattering measurements on lysozyme/D2O solutions in absence and in presence of the disaccharide. The findings emphasise the capability of trehalose to stabilise the protein, activating aggregationprocesses.
2007
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11570/1713629
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