Spectroscopic evidence of the effects induced by non-ionizing radiation on tissue samples

Aim of the present investigation is the study of the effects of the electromagnetic field (EMF) on biological samples, namely rat kidney and heart tissues, exposed to a 900 MHz non-ionizing radiation, by using the combination of two different spectroscopic methods, namely Fourier Transform Infrared (FT-IR) absorbance and optical microscopy. The FT-IR spectral features, collected in the C–H stretching and O–H stretching vibrational region (2500–3700 cm1), revealed consistent alterations passing from the control tissue samples to those irradiated. Emphasis is made on the high frequency O–H stretching band, for which a vibrational assignment of the observed sub-bands has been made. The spectroscopic results give quantitative information that are related to, and appear in full agreement with, the morphological observations carried out by optical microscopy measurements.