Free radical scavenging activity of the methanolic extract of the roots of Centaurea centaurium L. (Asteraceae) and its phenolic, non-polar and water fractions was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The phenolic fraction showed high radical-scavenging activity with DPPH (IC50 of 57 gmL-1) and potent antioxidant properties both on lipid peroxidation and beta-carotene bleaching (IC50 of 195 and 5 gmL-1, respectively). The n-hexane fraction of the extract showed the highest inhibitory activity against alpha-amylase with an IC50 of 158 gmL-1. In this fraction, the most abundant constituents were the fatty acids: 11,14-eicosadienoic acid methyl ester, 9-octadecenoic acid methyl ester and 9-octadecenoic acid. Various terpenes, e.g. cypirene, alpha-zingiberene, beta-farnesene, beta-santalene, beta-bisabolene, beta-himachalene and azulene were also present. The LC50 against the brine shrimp nauplii for the methanolic extract was found to be 44.05 gmL-1, whilst LC50 values for the polyphenolic, lipophilic and water fractions were found to be 157.44, 25.98 and 152.81 gmL-1,respectively.
Antioxidant, alpha-amylase inhibitory and brine-shrimp toxicity studies on Centaurea centaurium L. methanolic root extract
RAPISARDA, Antonio;
2008-01-01
Abstract
Free radical scavenging activity of the methanolic extract of the roots of Centaurea centaurium L. (Asteraceae) and its phenolic, non-polar and water fractions was measured using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The phenolic fraction showed high radical-scavenging activity with DPPH (IC50 of 57 gmL-1) and potent antioxidant properties both on lipid peroxidation and beta-carotene bleaching (IC50 of 195 and 5 gmL-1, respectively). The n-hexane fraction of the extract showed the highest inhibitory activity against alpha-amylase with an IC50 of 158 gmL-1. In this fraction, the most abundant constituents were the fatty acids: 11,14-eicosadienoic acid methyl ester, 9-octadecenoic acid methyl ester and 9-octadecenoic acid. Various terpenes, e.g. cypirene, alpha-zingiberene, beta-farnesene, beta-santalene, beta-bisabolene, beta-himachalene and azulene were also present. The LC50 against the brine shrimp nauplii for the methanolic extract was found to be 44.05 gmL-1, whilst LC50 values for the polyphenolic, lipophilic and water fractions were found to be 157.44, 25.98 and 152.81 gmL-1,respectively.Pubblicazioni consigliate
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