Little is known of how and where bacterial recognition triggers the induction of type I interferon. Whether the type of recognition receptor used in these responses is determined by the subcellular location of bacteria is not understood. Here we show that phagosomal bacteria such as group B streptococcus, but not cytosolic bacteria, potently induced interferon in conventional dendritic cells by a mechanism that required Toll-like receptor 7, the adaptor MyD88 and the transcription factor IRF1, all of which localized together with bacterial products in degradative vacuoles bearing lysosomal markers. Thus, this cell type–specific recognition pathway links lysosomal recognition of bacterial RNA with a robust, host-protective interferon response.

Bacterial recognition by TLR7 in the lysosomes of conventional dendritic cells

MANCUSO, Giuseppe;MIDIRI, Angelina;BIONDO, Carmelo;PAPASERGI, salvatore;TETI, Giuseppe;BENINATI, Concetta
2009-01-01

Abstract

Little is known of how and where bacterial recognition triggers the induction of type I interferon. Whether the type of recognition receptor used in these responses is determined by the subcellular location of bacteria is not understood. Here we show that phagosomal bacteria such as group B streptococcus, but not cytosolic bacteria, potently induced interferon in conventional dendritic cells by a mechanism that required Toll-like receptor 7, the adaptor MyD88 and the transcription factor IRF1, all of which localized together with bacterial products in degradative vacuoles bearing lysosomal markers. Thus, this cell type–specific recognition pathway links lysosomal recognition of bacterial RNA with a robust, host-protective interferon response.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11570/1900126
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