We have developed a supramolecular nanoassembly capable of inducing remarkable levels of cancer cell mortality through a bimodal action based on the simultaneous photogeneration of nitric oxide (NO) and singlet oxygen (1O2). This was achieved through the appropriate incorporation of an anionic porphyrin (as 1O2 photosensitizer) and of a tailored NO photodonor in different compartments of biocompatible nanoparticles based on cationic amphiphilic cyclodextrins. The combination of steady-state and timeresolved spectroscopic techniques showed the absence of significant intraand interchromophoric interaction between the two photoactive centers embedded in the nanoparticles, with consequent preservation of their photodynamic properties. Photodelivery of NO and 1O2 from the nanoassembly on visible light excitation was unambiguously demonstrated by direct and realtime monitoring of these transient species through amperometric and timeresolved infrared luminescence measurements, respectively. The typical red fluorescence of the porphyrin units was essentially unaffected in the bichromophoric nanoassembly, allowing its localization in living cells. The convergence of the dual therapeutic action and the imaging capacities in one single structure makes this supramolecular architecture an appealing, multifunctional candidate for applications in biomedical research
A Cyclodextrin-Based Nanoassembly with Bimodal Photodynamic Action
SCIORTINO, Maria Teresa;PARISI, TIZIANA;COLAO, IVANA;MAZZAGLIA, ANTONINO;
2012-01-01
Abstract
We have developed a supramolecular nanoassembly capable of inducing remarkable levels of cancer cell mortality through a bimodal action based on the simultaneous photogeneration of nitric oxide (NO) and singlet oxygen (1O2). This was achieved through the appropriate incorporation of an anionic porphyrin (as 1O2 photosensitizer) and of a tailored NO photodonor in different compartments of biocompatible nanoparticles based on cationic amphiphilic cyclodextrins. The combination of steady-state and timeresolved spectroscopic techniques showed the absence of significant intraand interchromophoric interaction between the two photoactive centers embedded in the nanoparticles, with consequent preservation of their photodynamic properties. Photodelivery of NO and 1O2 from the nanoassembly on visible light excitation was unambiguously demonstrated by direct and realtime monitoring of these transient species through amperometric and timeresolved infrared luminescence measurements, respectively. The typical red fluorescence of the porphyrin units was essentially unaffected in the bichromophoric nanoassembly, allowing its localization in living cells. The convergence of the dual therapeutic action and the imaging capacities in one single structure makes this supramolecular architecture an appealing, multifunctional candidate for applications in biomedical researchPubblicazioni consigliate
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