The effects of polychlorinated biphenyl congeners 126 and 153 (PCB-126 and PCB-153) on vitellogenin (Vtg) and cytochrome (CYP1A1) expression were evaluated in 60 juvenile Sparus aurata. Fish were divided into four groups and the control group (Group A) was compared to fish exposed to PCB-126 (10-8 M) (Group B), PCB-153 (10-6 M) (Group C) singly and also in combination (Group D) for 12, 24 and 72 h. Hepatic expression of Vtg and CYP1A1 were analyzed using histological examinations and by immunochemical (Western blotting and immunohistochemistry) methods. Vtg increased in juvenile fishes of Groups B, C and D after 12 hours respect to Group A and decreased after 24 and 72 h respect to 12 h in each group. CYP1A1 increased after 12 and 24 h in all groups vs control group and increased in Group B only at 72 h vs in control group. The results showed that chemical interaction and endocrine disruption in fish might produce deleterious consequences not only for fish but also for human.

Activation of the Ahr signalling pathway by polychlorobiphenyls causes a marked induction of cytochrome P450 only after depletion of vitellogenin in Sparus aurata

CALO', Margherita;CASELLA, stefania;PANZERA, Michele;LO CASCIO, Patrizia;ALBERGHINA, Daniela;PICCIONE, Giuseppe
2012

Abstract

The effects of polychlorinated biphenyl congeners 126 and 153 (PCB-126 and PCB-153) on vitellogenin (Vtg) and cytochrome (CYP1A1) expression were evaluated in 60 juvenile Sparus aurata. Fish were divided into four groups and the control group (Group A) was compared to fish exposed to PCB-126 (10-8 M) (Group B), PCB-153 (10-6 M) (Group C) singly and also in combination (Group D) for 12, 24 and 72 h. Hepatic expression of Vtg and CYP1A1 were analyzed using histological examinations and by immunochemical (Western blotting and immunohistochemistry) methods. Vtg increased in juvenile fishes of Groups B, C and D after 12 hours respect to Group A and decreased after 24 and 72 h respect to 12 h in each group. CYP1A1 increased after 12 and 24 h in all groups vs control group and increased in Group B only at 72 h vs in control group. The results showed that chemical interaction and endocrine disruption in fish might produce deleterious consequences not only for fish but also for human.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11570/2349049
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