The effects of microwaves (MWs) on the secondary structure of haemoglobin were investigated by means of Fourier Transform Infrared (FTIR) Spectroscopy. A set of samples of 250μl of different hemoglobin aqueous solutions (also in the presence of sucrose, trehalose and NaCl) were exposed for 3 hours to 900 MHz mobile phone MWs at a magnetic field intensity around 18 mA/m. Quantitative spectral analysis revealed significant increases in intensity of amide I and II modes after exposure to MWs for haemoglobin in bidistilled water, but not for hemoglobin in sodiumchloride, sucrose and trehalose aqueous solutions. Indeed, MWs interaction with the α-helix structure increases its dipole moment, produces increases in intensity of amide I and II modes whose major contribution is related to the α-helix content. This effect did not result to be significant for haemoglobin in sodium chloride, sucrose and trehalose aqueous solutions.

Microwave-induced increase of amide I and amide II vibration bands and modulating functions of sodium-chloride, sucrose and trehalose aqueous solutions: The case study of Haemoglobin

CALABRO', EMANUELE;MAGAZU', Salvatore;CAMPO, Salvatore Giuseppe
2012

Abstract

The effects of microwaves (MWs) on the secondary structure of haemoglobin were investigated by means of Fourier Transform Infrared (FTIR) Spectroscopy. A set of samples of 250μl of different hemoglobin aqueous solutions (also in the presence of sucrose, trehalose and NaCl) were exposed for 3 hours to 900 MHz mobile phone MWs at a magnetic field intensity around 18 mA/m. Quantitative spectral analysis revealed significant increases in intensity of amide I and II modes after exposure to MWs for haemoglobin in bidistilled water, but not for hemoglobin in sodiumchloride, sucrose and trehalose aqueous solutions. Indeed, MWs interaction with the α-helix structure increases its dipole moment, produces increases in intensity of amide I and II modes whose major contribution is related to the α-helix content. This effect did not result to be significant for haemoglobin in sodium chloride, sucrose and trehalose aqueous solutions.
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11570/2489021
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 25
  • ???jsp.display-item.citation.isi??? 17
social impact