The aim of this in vitro study was to evaluate the effect of sodium hypochlorite on the substantivity of chlorhexidine (CHX). Forty dentin tubes prepared from human maxillary central and lateral incisor teeth were used. Specimens were contaminated with Enterococcus faecalis for 14 days and were divided into five groups as follows: 2% CHX; 1.3% NaOCl; 2% CHX+1.3% NaOCl; infected dentin tubes (positive control); and sterile dentin tubes (negative control). Dentin chips were collected with round burs into brain heart infusion broth and after culturing, the number of colony-forming units (CFU) was counted. Data were analyzed using analysis of variance and covariance with repeated measures. Significance level was set at p< 0.05. The number of CFU was minimum in the first cultures in all experimental groups, and the results obtained were significantly different from each other at any time period (P < 0.05). At the first and second cultures the number of CFU in the CHX+NaOCl group was lower than two other groups. At the 14 day and 21 day experimental periods the CHX group showed the most effective antibacterial action. NaOCl group showed the worst result at third and fourth periods (P < 0.05). In each group, the number of CFU increased significantly by time-lapse (P < 0.05). In conclusion, NaOCl had no additive effect the residual antibacterial activity of CHX.

Effect of sodium hypochlorite on the substantivity of Chlorhexidine

LO GIUDICE, Giuseppe;
2013-01-01

Abstract

The aim of this in vitro study was to evaluate the effect of sodium hypochlorite on the substantivity of chlorhexidine (CHX). Forty dentin tubes prepared from human maxillary central and lateral incisor teeth were used. Specimens were contaminated with Enterococcus faecalis for 14 days and were divided into five groups as follows: 2% CHX; 1.3% NaOCl; 2% CHX+1.3% NaOCl; infected dentin tubes (positive control); and sterile dentin tubes (negative control). Dentin chips were collected with round burs into brain heart infusion broth and after culturing, the number of colony-forming units (CFU) was counted. Data were analyzed using analysis of variance and covariance with repeated measures. Significance level was set at p< 0.05. The number of CFU was minimum in the first cultures in all experimental groups, and the results obtained were significantly different from each other at any time period (P < 0.05). At the first and second cultures the number of CFU in the CHX+NaOCl group was lower than two other groups. At the 14 day and 21 day experimental periods the CHX group showed the most effective antibacterial action. NaOCl group showed the worst result at third and fourth periods (P < 0.05). In each group, the number of CFU increased significantly by time-lapse (P < 0.05). In conclusion, NaOCl had no additive effect the residual antibacterial activity of CHX.
2013
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11570/2649579
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