During sequencing of the genome of Clostridium perfringens strain 5147-97, a putative prophage was identified, located within a gene for a proposed flavodoxin oxidoreductase. Mitomycin C induction of this strain released a bacteriophage whose morphological features examined by electron microscopy indicated it belonged to the family Siphoviridae. This phage was hence designated as vB_CpeS-CP51. The 39,108-bp genome includes 50 predicted open reading frames (ORFs), including two that may affect sporulation, and two predicted tRNAs. To determine the ends of the prophage, PCR was performed using primers facing outwards from the proposed end genes. This confirmed the presence of a circularised genome in PEG-precipitated bacteriophage particles.

Complete genome sequence of ΦCP51, a temperate bacteriophage of Clostridium perfringens.

GERVASI, TERESA;
2013

Abstract

During sequencing of the genome of Clostridium perfringens strain 5147-97, a putative prophage was identified, located within a gene for a proposed flavodoxin oxidoreductase. Mitomycin C induction of this strain released a bacteriophage whose morphological features examined by electron microscopy indicated it belonged to the family Siphoviridae. This phage was hence designated as vB_CpeS-CP51. The 39,108-bp genome includes 50 predicted open reading frames (ORFs), including two that may affect sporulation, and two predicted tRNAs. To determine the ends of the prophage, PCR was performed using primers facing outwards from the proposed end genes. This confirmed the presence of a circularised genome in PEG-precipitated bacteriophage particles.
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11570/2671315
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 7
  • Scopus 15
  • ???jsp.display-item.citation.isi??? 13
social impact