A sensitive radioimmunoassay was developed for specific detection of IgM antibodies to the hepatitis B virus-associated δ antigen. The test is based on the selective absorption of IgM by anti-IgM fixed on a solid phase. Transient primary IgM anti-δ responses with no conversion to a secondary IgG response were observed in acute self-limited δ infection. IgM anti-δ was invariably found in hepatitis B surface antigen (HBsAg) carriers with active δ infection and liver disease, while it was absent in HBsAg-positive or negative individuals with anti-δ of IgG class but without liver damage or intrahepatic δ antigen. IgM anti-δ appears useful in defining the epidemiology of acute δ infection and in the serological diagnosis of active δ disease from nonpathogenic or past δ infection.
Radioimmunoassay detection of igm antibodies to the hbv-associated delta (δ) antigen: Clinical significance in δ infection
RAIMONDO, Giovanni;
1982-01-01
Abstract
A sensitive radioimmunoassay was developed for specific detection of IgM antibodies to the hepatitis B virus-associated δ antigen. The test is based on the selective absorption of IgM by anti-IgM fixed on a solid phase. Transient primary IgM anti-δ responses with no conversion to a secondary IgG response were observed in acute self-limited δ infection. IgM anti-δ was invariably found in hepatitis B surface antigen (HBsAg) carriers with active δ infection and liver disease, while it was absent in HBsAg-positive or negative individuals with anti-δ of IgG class but without liver damage or intrahepatic δ antigen. IgM anti-δ appears useful in defining the epidemiology of acute δ infection and in the serological diagnosis of active δ disease from nonpathogenic or past δ infection.Pubblicazioni consigliate
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