A novel actinobacterium, strain BC637T, was isolated from a biodeteriogenic biofilm sample 38 collected in 2009 in the Saint Callistus Roman catacombs. The strain was found to belong to 39 the genus Kribbella by analysis of the 16S rRNA gene. Phylogenetic analysis using the 16S 40 rRNA gene and the gyrB, rpoB, relA, recA and atpD concatenated gene sequences showed that strain BC637T 41 was most closely related to the type strains of Kribbella lupini and Kribbella endophytica. DNA-DNA hybridisation experiments confirmed that strain BC637T 42 is a distinct 43 genomic species from its closest phylogenetic relatives K. endophytica DSM 23718T (63% DNA relatedness) and K. lupini LU 14T 44 (63% DNA relatedness). Physiological comparisons 45 showed that strain BC637T is phenotypically distinct from the type strains of K. endophytica 46 and K. lupini. Strain BC637T is thus presented as the type strain of a novel species, for which the name Kribbella italica sp. nov. is proposed (= DSM 28967T = NRRL B-59155T47 ).
Description of Kribbella italica sp. nov., isolated from a Roman catacomb
DE LEO, FILOMENA;URZI', Clara Enza;
2015-01-01
Abstract
A novel actinobacterium, strain BC637T, was isolated from a biodeteriogenic biofilm sample 38 collected in 2009 in the Saint Callistus Roman catacombs. The strain was found to belong to 39 the genus Kribbella by analysis of the 16S rRNA gene. Phylogenetic analysis using the 16S 40 rRNA gene and the gyrB, rpoB, relA, recA and atpD concatenated gene sequences showed that strain BC637T 41 was most closely related to the type strains of Kribbella lupini and Kribbella endophytica. DNA-DNA hybridisation experiments confirmed that strain BC637T 42 is a distinct 43 genomic species from its closest phylogenetic relatives K. endophytica DSM 23718T (63% DNA relatedness) and K. lupini LU 14T 44 (63% DNA relatedness). Physiological comparisons 45 showed that strain BC637T is phenotypically distinct from the type strains of K. endophytica 46 and K. lupini. Strain BC637T is thus presented as the type strain of a novel species, for which the name Kribbella italica sp. nov. is proposed (= DSM 28967T = NRRL B-59155T47 ).Pubblicazioni consigliate
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