The aim of this research was to explain the direct plant growth-prornoting activity of Trichodenna harzianum strain T-22 (T22), hypothesizing the involvement of different c1asses of plant growth regulators. Seven days after the transfer to root-inducing rnediurn, in vitro-cultured shoots of GiSeLa6® (Prunus cerasus x P. canescens) were inoculated with T22. Root and shoot growth were significantly affecled by T22 (+76 and +61 %, respectively). Ten days after inoculation, the levels of indole-3-acetic acid (lAA), trans-zeatin riboside (t-ZR), dihydrozeatin riboside (DHZR), gibberellic acid (GA3) and abscisic acid (ABA) were analyzed by high performance liquid chromalography coupled with mass spectrometry. The resulls showed thal after T22-inoculation, 1AA and GA3 significantly increased in both leaves (+49 and +71%, respectively) and rools (+40 and +143%, respectively) whereas t-ZR decreased (-51% in leaves and -37% in rools). Changes in DHZR were observed in T22- inoculated roots (-32%) but not in leaves, whereas the levels of ABA clid noI cliffer belween the lwo treatruenls. The extraction method allowed the simultaneous extraction of phytohormones. There is evidence that the change in phytohormone levels is one of the direct mechanism by which T22 promoles rooting and shool growth, with notable advantages for rootstock production during nursery processes.
Trichoderma harzianum strain T-22 induces changes in phytohormone levels in cherry rootstocks (Prunus cerasus × P. canescens)
DE NISCO, Mauro;
2011-01-01
Abstract
The aim of this research was to explain the direct plant growth-prornoting activity of Trichodenna harzianum strain T-22 (T22), hypothesizing the involvement of different c1asses of plant growth regulators. Seven days after the transfer to root-inducing rnediurn, in vitro-cultured shoots of GiSeLa6® (Prunus cerasus x P. canescens) were inoculated with T22. Root and shoot growth were significantly affecled by T22 (+76 and +61 %, respectively). Ten days after inoculation, the levels of indole-3-acetic acid (lAA), trans-zeatin riboside (t-ZR), dihydrozeatin riboside (DHZR), gibberellic acid (GA3) and abscisic acid (ABA) were analyzed by high performance liquid chromalography coupled with mass spectrometry. The resulls showed thal after T22-inoculation, 1AA and GA3 significantly increased in both leaves (+49 and +71%, respectively) and rools (+40 and +143%, respectively) whereas t-ZR decreased (-51% in leaves and -37% in rools). Changes in DHZR were observed in T22- inoculated roots (-32%) but not in leaves, whereas the levels of ABA clid noI cliffer belween the lwo treatruenls. The extraction method allowed the simultaneous extraction of phytohormones. There is evidence that the change in phytohormone levels is one of the direct mechanism by which T22 promoles rooting and shool growth, with notable advantages for rootstock production during nursery processes.Pubblicazioni consigliate
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