Porphyromonas gingivalis biofilm formation in different titanium surfaces, an in vitro study

Objective:The aim of this work was to evaluate the biofilm formation ofPorphyromonasgingivalison disks of titanium (Ti) grade 4 (G4) and Ti-6Al-4V alloy grade 5 (G5) with differentsurface topographies.Materials and methods:Porphyromonas gingivalisATCC 33277 was used to develop anin vitromature biofilm on a total of 96 disk-shaped specimens of laser-treated (L), sandblasted (S), andmachined (M) surfaces of Ti G4 and Ti G5. Surface roughness (Ra) and the wettability contact angle(WCA) were measured to characterize the surface of the specimens. The bacterial biofilm wasevaluated by biomass quantification, bacterial viability, visualization of the biofilm extracellularmatrix, and bacterial cell count. Data were analyzed using one-way ANOVA and Holm–Sidak testsand expressed as meanstandard deviation.Results:The Ra for the L group was 0.10 (0.07)lm inside the craters and 0.40 (0.08)lm in thearea surrounding the craters resulting the smoothest (P<0.05) in respect to the S group(1.300.61lm) and the M group (0.750.23lm). The L group showed a higher WCA than Sand M groups for both G4 (109.9°6.6) and G5 (104.2°5.9) materials (P<0.05). The L groupdisplayed both the lessP. gingivalisbacterial biomass (0.380.01 for G4; 0.620.02 for G5) thatwas significant in respect to G4-S (P<0.001), G4-M (P<0.001), and G5-M (P=0.001) and the lesstotal cell number (21518 for G4 and 2449 for G5) than S and M groups for both G4 and G5materials (P<0.01).Conclusion:Within the limits of the present study, the results showed that G4-L appears to besignificantly efficient in the reduction of theP. gingivalisbiofilm formation.