In this paper we describe a novel portable system for nucleic acids electrochemical detection. The core of the sys- tem is a miniaturized silicon chip composed by planar microelectrodes. The chip is embedded on PCB board for the electrical driving and reading. The counter, reference and work microelectrodes are manufactured using the VLSI technology, the material is gold for reference and counter electrodes and platinum for working electrode. The device contains also a resistor to control and measuring the temperature for PCR thermal cycling. The reac- tion chamber has a total volume of 20 μL. It is made in hybrid silicon–plastic technology. Each device contains four independent electrochemical cells. Results show HBV Hepatitis-B virus detection using an unspecific DNA intercalating redox probe based on metal– organic compounds. The recognition event is sensitively detected by square wave voltammetry monitoring the redox signals of the intercalator that strongly binds to the double-stranded DNA. Two approaches were here eval- uated: (a) intercalation of electrochemical unspecific probe on ds-DNA on homogeneous solution (homogeneous phase); (b) grafting of DNA probes on electrode surface (solid phase). The system and the method here reported offer better advantages in term of analytical performances compared to the standard commercial optical-based real-time PCR systems, with the additional incomes of being potential- ly cheaper and easier to integrate in a miniaturized device.

A miniaturized silicon based device for nucleic acids electrochemical detection

PUNTORIERO, Fausto;TROVATO, EMANUELA;Conoci, Sabrina
2015

Abstract

In this paper we describe a novel portable system for nucleic acids electrochemical detection. The core of the sys- tem is a miniaturized silicon chip composed by planar microelectrodes. The chip is embedded on PCB board for the electrical driving and reading. The counter, reference and work microelectrodes are manufactured using the VLSI technology, the material is gold for reference and counter electrodes and platinum for working electrode. The device contains also a resistor to control and measuring the temperature for PCR thermal cycling. The reac- tion chamber has a total volume of 20 μL. It is made in hybrid silicon–plastic technology. Each device contains four independent electrochemical cells. Results show HBV Hepatitis-B virus detection using an unspecific DNA intercalating redox probe based on metal– organic compounds. The recognition event is sensitively detected by square wave voltammetry monitoring the redox signals of the intercalator that strongly binds to the double-stranded DNA. Two approaches were here eval- uated: (a) intercalation of electrochemical unspecific probe on ds-DNA on homogeneous solution (homogeneous phase); (b) grafting of DNA probes on electrode surface (solid phase). The system and the method here reported offer better advantages in term of analytical performances compared to the standard commercial optical-based real-time PCR systems, with the additional incomes of being potential- ly cheaper and easier to integrate in a miniaturized device.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11570/3102059
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