An innovative chemical strategy integrated in a miniaturized electrochemical device was developed for sensitive detection of a pathogen genome (HBV virus) without any amplification step. The results show a limit of detection comparable to the standard qRT-PCR method (20 copies per reaction), paving the way to future development of genetic PoC devices addressing automatized and low-cost molecular diagnostics.
An innovative chemical strategy for PCR-free genetic detection of pathogens by an integrated electrochemical biosensor
Sciuto, E L;Di Pietro, M L;Conoci, S
Ultimo
2017-01-01
Abstract
An innovative chemical strategy integrated in a miniaturized electrochemical device was developed for sensitive detection of a pathogen genome (HBV virus) without any amplification step. The results show a limit of detection comparable to the standard qRT-PCR method (20 copies per reaction), paving the way to future development of genetic PoC devices addressing automatized and low-cost molecular diagnostics.File in questo prodotto:
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Descrizione: An innovative chemical strategy for PCR-free genetic detection of pathogens by an integrated electrochemical biosensor
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