Clinical evidences, experimental models, and epidemiology of many studies suggest that phlhalate-based plaslicizers, aliphalic eSler, and bisphenol A (BPA) have major risks for humans by targeting different organs and body systems. The current study has been designed frrstly to analyze three categones of cheese with and without their exposure to the sun and packed in packages with an inner surfacc plastic-covered film in order to identilY the dibutyl phthalate (DBP); benzyl butyl phthalate (BBP); bis(2-cthylhexyl) phthalate (DEHP); diisodecyl phthalate (DiDP); diisononyl phthalate (DiNP); and 1,2-cyclohexane dicarboxylic acid diisononyl ester (DINCH) by GC-MS/MS, except for the bisphcnol A, which is by UPLC-MS/MS, and secondly to assess the toxicity of the identified chemical molecules and cheese samples on the liver and kidney ofmice. Our results showed that the cheese contains high quantities ofDBP and DEHP with the concentrations up to 0.46 and 2.339 mg!kg, respectively. Other types of cheese, such as rolled and triangular cheeses, contain Iittle quantities ofthe ali substances at concentrations below tlle standard Iimits. In vivo, the obtained data clearly demonstrated that the acute administration ofDBP, DEHP, and the tested cheese significantly induced liver and kidney injunes in mice manifested by a rise in plasma alanine aminotransferase, aspartate aminolransferase, blood urea nitrogen, lactate dehydrogenase. urea, ereatinine, and une acid when compared with control animals. In addition, the histopathological study confll1ned the perturbation of biochemical parameters and showed that the hepatic and renal structures werc altered. lndeed, the hepatotoxic and nephrotoxic effects are more pronounced when cheese was exposed to the sun.

Persistent plasticizers and bisphenol in the cheese of Tunisian markets induced biochemical and histopathological alterations in male BALB/c mice

G. Di Bella
Supervision
;
2018-01-01

Abstract

Clinical evidences, experimental models, and epidemiology of many studies suggest that phlhalate-based plaslicizers, aliphalic eSler, and bisphenol A (BPA) have major risks for humans by targeting different organs and body systems. The current study has been designed frrstly to analyze three categones of cheese with and without their exposure to the sun and packed in packages with an inner surfacc plastic-covered film in order to identilY the dibutyl phthalate (DBP); benzyl butyl phthalate (BBP); bis(2-cthylhexyl) phthalate (DEHP); diisodecyl phthalate (DiDP); diisononyl phthalate (DiNP); and 1,2-cyclohexane dicarboxylic acid diisononyl ester (DINCH) by GC-MS/MS, except for the bisphcnol A, which is by UPLC-MS/MS, and secondly to assess the toxicity of the identified chemical molecules and cheese samples on the liver and kidney ofmice. Our results showed that the cheese contains high quantities ofDBP and DEHP with the concentrations up to 0.46 and 2.339 mg!kg, respectively. Other types of cheese, such as rolled and triangular cheeses, contain Iittle quantities ofthe ali substances at concentrations below tlle standard Iimits. In vivo, the obtained data clearly demonstrated that the acute administration ofDBP, DEHP, and the tested cheese significantly induced liver and kidney injunes in mice manifested by a rise in plasma alanine aminotransferase, aspartate aminolransferase, blood urea nitrogen, lactate dehydrogenase. urea, ereatinine, and une acid when compared with control animals. In addition, the histopathological study confll1ned the perturbation of biochemical parameters and showed that the hepatic and renal structures werc altered. lndeed, the hepatotoxic and nephrotoxic effects are more pronounced when cheese was exposed to the sun.
2018
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11570/3118090
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