Serotonin (5-hydroxytryptamine [5-HT]) may induce metabolic effects in different cell types, including leukocytes. In horses, 5-HT is involved in physiological and behavioral functions. Physical exercise is known to increase the amounts of 5-HT both in brain and periphery, but so far, the signal mechanism in response to exercise is not known. The aim of the study was to investigate the effect of a racehorse intensive training session on plasma 5-HT levels, serotonin transporter (SERT), 5HT1A, 5-HT2A, 5-HT1B, 5-HT7 receptor, interleukin-1 beta, and tumor necrosis factor-alpha expression in horse peripheral blood mononuclear cells (PBMC). In particular, the research was carried out on 12 trained horses performing daily training. Plasma 5-HT levels were analyzed in platelet-poor plasma fraction by enzyme-linked immunosorbent assay at T0, T1, and T2 (pretraining, 30 minutes post-training, and 2 hours post-training session), respectively. Peripheral blood mononuclear cells were isolated to perform real-time polymerase chain reaction for the evaluation of SERT, 5-HT receptor, and cytokine mRNA levels. The results showed significantly increased levels of plasma 5-HT, 5HT1A, and 5-HT2A and significantly decreased levels of SERT, 5-HT1B, 5-HT7, and both cytokine mRNAs in PBMC at T1, compared with T0 and T2. The results were confirmed by in vitro experiment. Training may induce a lower degree of 5-HT storage and, therefore, a higher plasma 5-HT concentrations. Leukocyte 5-HT receptor mRNAs seem strongly influenced by the exercise. Observed changes suggest a transient neuroendocrinological response to the exercise. A better understanding of the influence of physical exercise on serotoninergic system could have potential application for the implementation of training protocols in racing horses.

Physical exercise affects serotoninergic system in horse leucocytes

Bruschetta G.
Primo
;
d’Ascola A.
Secondo
;
Medica P.
Penultimo
;
Ferlazzo A. M.
Ultimo
2020

Abstract

Serotonin (5-hydroxytryptamine [5-HT]) may induce metabolic effects in different cell types, including leukocytes. In horses, 5-HT is involved in physiological and behavioral functions. Physical exercise is known to increase the amounts of 5-HT both in brain and periphery, but so far, the signal mechanism in response to exercise is not known. The aim of the study was to investigate the effect of a racehorse intensive training session on plasma 5-HT levels, serotonin transporter (SERT), 5HT1A, 5-HT2A, 5-HT1B, 5-HT7 receptor, interleukin-1 beta, and tumor necrosis factor-alpha expression in horse peripheral blood mononuclear cells (PBMC). In particular, the research was carried out on 12 trained horses performing daily training. Plasma 5-HT levels were analyzed in platelet-poor plasma fraction by enzyme-linked immunosorbent assay at T0, T1, and T2 (pretraining, 30 minutes post-training, and 2 hours post-training session), respectively. Peripheral blood mononuclear cells were isolated to perform real-time polymerase chain reaction for the evaluation of SERT, 5-HT receptor, and cytokine mRNA levels. The results showed significantly increased levels of plasma 5-HT, 5HT1A, and 5-HT2A and significantly decreased levels of SERT, 5-HT1B, 5-HT7, and both cytokine mRNAs in PBMC at T1, compared with T0 and T2. The results were confirmed by in vitro experiment. Training may induce a lower degree of 5-HT storage and, therefore, a higher plasma 5-HT concentrations. Leukocyte 5-HT receptor mRNAs seem strongly influenced by the exercise. Observed changes suggest a transient neuroendocrinological response to the exercise. A better understanding of the influence of physical exercise on serotoninergic system could have potential application for the implementation of training protocols in racing horses.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11570/3157811
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