The combination of cyclodextrins (CD) with graphene-based nanomaterials (GBN) has been widely investigated to obtain graphene/cyclodextrin nanomaterials (GCD) endowed with unique relationships with biomolecules and/or living cells. However, their development in nanomedicine is still confined in the laboratory arena due to the lack of in vivo studies and safety data. Here, we report the synthesis and physicochemical properties of a new fluorescent graphene platform obtained by supramolecular assembly of cyclodextrin-functionalized graphene (GCD) and adamantanyl-rhodamine derivative (AdaRhod-2). The safety issues of GCD were assessed by in vitro cell culture and in vivo by a zebrafish embryo toxicity test (ZFET) to evaluate possible toxicity and pathological lesions of zebrafish (ZF) exposed to GCD@AdaRhod-2 at two selected concentrations 4 mg/L and 80 mg/L. GCD@AdaRhod-2 showed a reduced potential risk with respect to other GBN with a survival rate percentage more than 80% for both investigated concentrations. Finally, the fate of GCD@AdaRhod-2 during ZF embryo development was monitored by confocal microscopy images.

In vivo tracking and biosafety of fluorescent graphene-cyclodextrin nanomaterials on zebrafish embryos

Neri G.
Primo
;
Iaria C.
Secondo
;
Capparucci F.;Capillo G.;Pennisi R.;Scala A.;Maria Teresa Sciortino
Writing – Original Draft Preparation
;
Piperno A.
Penultimo
Writing – Original Draft Preparation
;
Marino F.
Ultimo
Writing – Original Draft Preparation
2022-01-01

Abstract

The combination of cyclodextrins (CD) with graphene-based nanomaterials (GBN) has been widely investigated to obtain graphene/cyclodextrin nanomaterials (GCD) endowed with unique relationships with biomolecules and/or living cells. However, their development in nanomedicine is still confined in the laboratory arena due to the lack of in vivo studies and safety data. Here, we report the synthesis and physicochemical properties of a new fluorescent graphene platform obtained by supramolecular assembly of cyclodextrin-functionalized graphene (GCD) and adamantanyl-rhodamine derivative (AdaRhod-2). The safety issues of GCD were assessed by in vitro cell culture and in vivo by a zebrafish embryo toxicity test (ZFET) to evaluate possible toxicity and pathological lesions of zebrafish (ZF) exposed to GCD@AdaRhod-2 at two selected concentrations 4 mg/L and 80 mg/L. GCD@AdaRhod-2 showed a reduced potential risk with respect to other GBN with a survival rate percentage more than 80% for both investigated concentrations. Finally, the fate of GCD@AdaRhod-2 during ZF embryo development was monitored by confocal microscopy images.
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11570/3237888
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
social impact