NT-PROBNP assay and MINE score in dogs with mitral valve disease

Myxomatous mitral valve disease (MMVD) is the most common acquired cardiac disease in dogs. This study aimed to evaluate the relationship between the Mitral INsufficiency Echocardiographic (MINE) score, an echocardiographic staging system, and plasma NT-proBNP levels. Twenty-one client-owned dogs with MMVD were prospectively enrolled and underwent clinical, radiographic, electrocardiographic, and echocardiographic examinations. Dogs were classified according to MINE score. Plasma NT-proBNP concentrations were measured using a validated immunoassay. Results showed significant differences in echocardiographic parameters among severity groups and demonstrated a correlation between disease severity and NT-proBNP levels. These findings suggest that combining the MINE score with NT-proBNP measurement may improve staging accuracy, the association with MMVD severity, and clinical management of dogs affected by MMVD.