In this study, a rapid separation of eleven polyphenols and three methylxanthines was obtained by means of nano-liquid chromatography (nano-LC), employing a 100 mu m I.D. capillary column packed with C18 core-shell particles (2.7 mu m) for 10 cm. All compounds were baseline resolved with a step gradient elution in less than 15 min. The developed analytical method was validated and the resulting RSD% for intra-day and inter-day repeatability, related to retention time, retention factor and peak area, were below 5.1 and 5.7%. LOD and LOQ values corresponded to 0.300 and 0.625 mu g/mL, while linearity range assessed gave R-2 no lower than 0.990. Then, the method was used to determine studied compounds in tea extracts. Further, the nano-LC system was coupled with a mass spectrometer to confirm the components present in real samples. Finally the results were compared with those obtained using a capillary column (100 mu m I.D. x 10 cm) packed with C18 sub-2 mu m particles, applying the same nano-LC experimental conditions. (C) 2012 Elsevier B.V. All rights reserved.
Analysis of polyphenols and methylxantines in tea samples by means of nano-liquid chromatography utilizing capillary columns packed with core-shell particles
MONDELLO, Luigi;
2012-01-01
Abstract
In this study, a rapid separation of eleven polyphenols and three methylxanthines was obtained by means of nano-liquid chromatography (nano-LC), employing a 100 mu m I.D. capillary column packed with C18 core-shell particles (2.7 mu m) for 10 cm. All compounds were baseline resolved with a step gradient elution in less than 15 min. The developed analytical method was validated and the resulting RSD% for intra-day and inter-day repeatability, related to retention time, retention factor and peak area, were below 5.1 and 5.7%. LOD and LOQ values corresponded to 0.300 and 0.625 mu g/mL, while linearity range assessed gave R-2 no lower than 0.990. Then, the method was used to determine studied compounds in tea extracts. Further, the nano-LC system was coupled with a mass spectrometer to confirm the components present in real samples. Finally the results were compared with those obtained using a capillary column (100 mu m I.D. x 10 cm) packed with C18 sub-2 mu m particles, applying the same nano-LC experimental conditions. (C) 2012 Elsevier B.V. All rights reserved.Pubblicazioni consigliate
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