Phospholipids (PLs) comprise an important kind of amphiphilic molecules, with lipophilic acyl chains and a hydrophilic head. These compounds possess important physiological functions, as well as positive nutritional properties. Recent studies have in fact given considerable evidence that PLs can exert beneficial effects on human health, such as anti-inflammatory activity and reduction of the risk of cardiovascular disease. PLs are also used as emulsifiers or emulsion stabilisers in the food industry, in the form of complex with proteins. Five major classes of phospholipids are usually found in milk fat, and they are: phosphatidylcholine (PC), phosphatidylethanolamine (PE), sphingomyelin (SM), phosphatidylinositol (PI) and phosphatidylserine (PS). Phospholipids are located on the milk fat globule membrane (MFGM), where they contribute significantly to the emulsification role of the membrane by virtue of both lipophilic and hydrophilic properties [1]. The aim of this study was to determine the PLs content of Folch and SPE extracted milk samples from different animals such as cow and donkey. Hydrophilic interaction liquid chromatography (HILIC) was used to achieve baseline separation of major PLs classes in various milk samples. Evaporative light scattering detection (ELSD) was further employed to attain the quantitative evaluation of major PLs classes identified, achieved by external calibration. By interpolation of the calibration curves, major classes of PLs identified the Folch and SPE-extracted samples were quantified. ESI-MS detection was used to identify PLs classes and to determine the presence of single PL within each class.Extraction and chromatographic methods were validated.
DETERMINATION OF PHOSPHOLIPIDS IN VARIOUS MILK SAMPLES BY MEANS OF HILIC-ELSD/MS
CICHELLO, FILOMENA;RUSSO, marina;SOMMELLA, EDUARDO MARIA;DONATO, Paola Agata Eustochia;TRANCHIDA, Peter Quinto;DUGO, Giovanni;DUGO, Paola;MONDELLO, Luigi
2012-01-01
Abstract
Phospholipids (PLs) comprise an important kind of amphiphilic molecules, with lipophilic acyl chains and a hydrophilic head. These compounds possess important physiological functions, as well as positive nutritional properties. Recent studies have in fact given considerable evidence that PLs can exert beneficial effects on human health, such as anti-inflammatory activity and reduction of the risk of cardiovascular disease. PLs are also used as emulsifiers or emulsion stabilisers in the food industry, in the form of complex with proteins. Five major classes of phospholipids are usually found in milk fat, and they are: phosphatidylcholine (PC), phosphatidylethanolamine (PE), sphingomyelin (SM), phosphatidylinositol (PI) and phosphatidylserine (PS). Phospholipids are located on the milk fat globule membrane (MFGM), where they contribute significantly to the emulsification role of the membrane by virtue of both lipophilic and hydrophilic properties [1]. The aim of this study was to determine the PLs content of Folch and SPE extracted milk samples from different animals such as cow and donkey. Hydrophilic interaction liquid chromatography (HILIC) was used to achieve baseline separation of major PLs classes in various milk samples. Evaporative light scattering detection (ELSD) was further employed to attain the quantitative evaluation of major PLs classes identified, achieved by external calibration. By interpolation of the calibration curves, major classes of PLs identified the Folch and SPE-extracted samples were quantified. ESI-MS detection was used to identify PLs classes and to determine the presence of single PL within each class.Extraction and chromatographic methods were validated.Pubblicazioni consigliate
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