In the present research, similar chromatography fingerprints were obtained using finely-tuned cryogenic-modulation (CM) and flow-modulation (FM) comprehensive two-dimensional gas chromatography–mass spectrometry (GC×GC– MS) experimental conditions. The CM applications were carried out by using a loop-type modulator while the FM ones were performed using a seven-port wafer chip. The CM GC×GC–MS column combination consisted of a low-polarity 30 m × 0.25 mm i.d. × 0.25-μm df column and a mid-polarity 1.5 m × 0.25 mm i.d. × 0.25-μm df one. With regard to the FM GC×GC–MS column combination, the same stationary phases were used, coated on a 20 m × 0.18 mm i.d. × 0.18-μm df column and a 5 m × 0.32 mm i.d. × 0.25-μm df one. A mixture of 64 cosmetic allergens was employed to measure different chromatography parameters, such as peak widths, resolution and signal-to-noise ratios. It is noteworthy that the FM combination of columns has been proposed as a standard column set, and the results herein reported confirm those previously attained (on a sample of bio-oil) in recent research.
Cryogenic-and flow-modulation comprehensive two-dimensional gas chromatography– mass spectrometry: Obtaining similar chromatographic performance
Giocastro B.;Aloisi I.;Zoccali M.
;Tranchida P. Q.;Mondello L.
2020-01-01
Abstract
In the present research, similar chromatography fingerprints were obtained using finely-tuned cryogenic-modulation (CM) and flow-modulation (FM) comprehensive two-dimensional gas chromatography–mass spectrometry (GC×GC– MS) experimental conditions. The CM applications were carried out by using a loop-type modulator while the FM ones were performed using a seven-port wafer chip. The CM GC×GC–MS column combination consisted of a low-polarity 30 m × 0.25 mm i.d. × 0.25-μm df column and a mid-polarity 1.5 m × 0.25 mm i.d. × 0.25-μm df one. With regard to the FM GC×GC–MS column combination, the same stationary phases were used, coated on a 20 m × 0.18 mm i.d. × 0.18-μm df column and a 5 m × 0.32 mm i.d. × 0.25-μm df one. A mixture of 64 cosmetic allergens was employed to measure different chromatography parameters, such as peak widths, resolution and signal-to-noise ratios. It is noteworthy that the FM combination of columns has been proposed as a standard column set, and the results herein reported confirm those previously attained (on a sample of bio-oil) in recent research.Pubblicazioni consigliate
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