Two-dimensional liquid chromatography is a technique of great analytical impact for handling very complex real-world samples. Principally based on the use of two orthogonal stationary phases, the separation power of a two-dimensional liquid chromatography system is greatly enhanced when compared to a conventional one-dimensional separation. In this chapter, general principles and theoretical/practical aspects of both heart-cutting and comprehensive two-dimensional liquid chromatography coupled to mass spectrometry methods are described. Significant applications in the field of downscaled miniaturized systems, viz. capillary liquid chromatography, are also reported and are mainly focused on the development of innovative strategies in proteomics studies.
Hyphenations of 2D capillary-based LC with mass spectrometry
Cacciola F.;Mangraviti D.;Mondello L.;Dugo P.
2019-01-01
Abstract
Two-dimensional liquid chromatography is a technique of great analytical impact for handling very complex real-world samples. Principally based on the use of two orthogonal stationary phases, the separation power of a two-dimensional liquid chromatography system is greatly enhanced when compared to a conventional one-dimensional separation. In this chapter, general principles and theoretical/practical aspects of both heart-cutting and comprehensive two-dimensional liquid chromatography coupled to mass spectrometry methods are described. Significant applications in the field of downscaled miniaturized systems, viz. capillary liquid chromatography, are also reported and are mainly focused on the development of innovative strategies in proteomics studies.Pubblicazioni consigliate
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