tIn this work, ultra high performance liquid chromatography was used for the characterization of non polar lipids (triacylglycerols) in milk samples of different origin, as well as milk-derivatives. For tackling sucha task, three core shell type octadecylsilica columns were serially coupled, reaching a total stationaryphase length of 45 cm, using acetonitrile-isopropanol gradient elution allowing triacylglicerol separa-tion according to increasing partition number. The employment of an ion-trap-time-of-flight detectionin conjunction with atmospheric-pressure chemical ionization mass spectrometry was carried out topositively identify a number of 243 different triacylglycerols containing up to 22 fatty acids, with 2-22carbon atom alkyl chain length, and 0-3 double bonds. This work reports an extensive characterization of the triacylglycerol fraction in milk and milk-related samples of different sources.

High performance characterization of triacylglycerols in milk and milk-related samples by liquid chromatography and mass spectrometry

BECCARIA, MARCO;CACCIOLA, FRANCESCO;DONATO, Paola Agata Eustochia;DUGO, Paola;MONDELLO, Luigi
2014-01-01

Abstract

tIn this work, ultra high performance liquid chromatography was used for the characterization of non polar lipids (triacylglycerols) in milk samples of different origin, as well as milk-derivatives. For tackling sucha task, three core shell type octadecylsilica columns were serially coupled, reaching a total stationaryphase length of 45 cm, using acetonitrile-isopropanol gradient elution allowing triacylglicerol separa-tion according to increasing partition number. The employment of an ion-trap-time-of-flight detectionin conjunction with atmospheric-pressure chemical ionization mass spectrometry was carried out topositively identify a number of 243 different triacylglycerols containing up to 22 fatty acids, with 2-22carbon atom alkyl chain length, and 0-3 double bonds. This work reports an extensive characterization of the triacylglycerol fraction in milk and milk-related samples of different sources.
2014
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11570/3046573
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